The Agilent 1290 Infinity II LC System represents a cutting-edge advancement in ultra-high performance liquid chromatography (UHPLC)‚ offering unparalleled speed‚ resolution‚ and sensitivity for modern analytical demands.
1.1 Overview of Ultra-High Performance Liquid Chromatography (UHPLC)
Ultra-High Performance Liquid Chromatography (UHPLC) is a advanced analytical technique that operates at extremely high pressures‚ enabling the use of small particle-sized columns for superior separation efficiency. It offers faster analysis times‚ higher resolution‚ and improved sensitivity compared to conventional HPLC‚ making it ideal for complex sample analysis in various fields.
1.2 Key Features of the Agilent 1290 Infinity II System
The Agilent 1290 Infinity II LC System features advanced UHPLC technology with exceptional speed and precision; It includes a high-speed pump delivering up to 1300 bar‚ a versatile multisampler‚ and a range of detectors for flexibility. The system is designed for seamless integration with advanced software‚ enabling high-throughput analysis and robust performance across diverse applications.
System Components and Modules
The Agilent 1290 Infinity II LC System comprises advanced modules‚ including the multisampler‚ high-speed pump‚ diode array detector‚ and MS flow modulator‚ ensuring high performance and flexibility.
2.1 Multisampler (G7167B)
The Agilent 1290 Infinity II Multisampler (G7167B) is designed for efficient sample handling‚ accommodating both vials and microtiter plates. It operates seamlessly at pressures up to 1300 bar‚ ensuring optimal chromatographic performance. The module’s stackable design enhances system integration‚ while its automation capabilities streamline workflows‚ making it ideal for high-throughput UHPLC applications.
2.2 High-Speed Pump (G7104A)
The Agilent 1290 Infinity II High-Speed Pump (G7104A) delivers exceptional performance with dual high-pressure pumps‚ enabling precise solvent delivery. It supports pressures up to 1200 bar and features a dual-channel solvent degasser for efficient operation. The pump integrates seamlessly with the system‚ ensuring high throughput and maintaining chromatographic integrity across a wide range of applications.
2.3 Diode Array Detector (DAD)
The Diode Array Detector (DAD) in the Agilent 1290 Infinity II system offers high optical performance and versatility. It provides full spectrum acquisition across a broad wavelength range‚ enabling simultaneous detection of multiple compounds. With features like a built-in holmium oxide filter for wavelength accuracy‚ the DAD ensures precise and reliable detection‚ meeting GLP compliance and facilitating method development. This makes it ideal for diverse analytical applications.
2.4 Variable Wavelength Detector (VWD)
The Variable Wavelength Detector (VWD) in the Agilent 1290 Infinity II system delivers high sensitivity and flexibility. It supports a wide wavelength range‚ enabling detection at specific wavelengths for optimal analyte detection. Designed for high-speed scanning and precise control‚ the VWD ensures compatibility with various chromatographic methods. Its robust design and easy maintenance make it suitable for demanding laboratory environments‚ enhancing overall system performance and reliability.
The MS Flow Modulator (G7170B) is a critical component of the Agilent 1290 Infinity II system‚ designed to optimize liquid chromatography-tandem mass spectrometry (LC-MS) workflows. It ensures precise flow control and efficient solvent delivery to the mass spectrometer‚ enhancing sensitivity and data quality. Compact and easy to integrate‚ this module supports high-throughput analysis while maintaining compatibility with various MS systems for seamless operation. The Agilent 1290 Infinity II LC System requires proper site preparation and adherence to specifications for successful installation. Mount modules securely‚ connect components‚ and follow guidelines for optimal setup and performance. The Agilent 1290 Infinity II LC System requires a stable environment with controlled temperature (15-25°C) and humidity (50-70%). Ensure electrical power meets specifications (100-240V AC‚ 50/60Hz). The system must be installed on a vibration-free surface and connected to a high-purity water supply. Proper ventilation and networking capabilities are essential for optimal performance and data integration. Begin by unpacking and inspecting all components. Connect the multisampler‚ high-speed pump‚ and detectors to the main system. Install InfinityLab software and perform initial calibration. Refer to the manual for detailed instructions on module integration and system setup. Ensure all connections are secure and power is supplied. Complete the installation by running a diagnostic test to verify system functionality. Regular maintenance ensures optimal performance. Clean detectors‚ replace seals‚ and inspect tubing. Troubleshooting guides in the manual help resolve common issues swiftly‚ minimizing downtime. Regular maintenance is crucial for optimal performance. Clean the detectors‚ replace worn seals‚ and inspect tubing for wear. Check pump performance and flush lines with recommended solvents. Schedule software updates and ensure all modules are aligned. Refer to the manual for detailed procedures and intervals to maintain system efficiency and extend instrument lifespan. Common issues include pressure fluctuations‚ detector noise‚ and leakage. Check tubing connections and replace worn parts. For noise‚ verify lamp stability and clean the detector flow cell. Leaks can be resolved by tightening fittings or replacing seals. Refer to the troubleshooting guide for detailed solutions to ensure system stability and performance. Always follow maintenance schedules. The Agilent 1290 Infinity II LC System ensures efficient operation with intuitive software control‚ enabling seamless method development and optimization for diverse chromatographic applications. The Agilent 1290 Infinity II LC System operates seamlessly with the InfinityLab software‚ enabling quick method execution. Users can easily prepare and run samples by selecting predefined methods‚ setting injection volumes‚ and specifying wavelengths. The system initializes the pump‚ equilibrates the column‚ and performs injections automatically. Real-time data monitoring and reporting tools ensure efficient analysis; Post-run‚ the system shuts down safely‚ maintaining instrument integrity and readiness for the next operation. The Agilent 1290 Infinity II LC System supports advanced method development through intuitive software tools. Users can optimize parameters like gradient profiles‚ column selection‚ and detection settings to achieve superior separation efficiency. The system also enables rapid method scouting and validation‚ ensuring robust and reproducible results. Automated optimization features streamline method fine-tuning‚ enhancing productivity in demanding analytical environments. The Agilent 1290 Infinity II LC System adheres to strict safety protocols and regulatory standards. Proper handling of modules and solvents is essential to ensure safe operation and compliance. Handling the Agilent 1290 Infinity II LC System requires adherence to specific safety measures to prevent injury and ensure optimal performance. Always wear protective gear when handling high-pressure components and corrosive solvents. Follow the recommended guidelines for module installation and maintenance to avoid system damage and ensure compliance with safety regulations. Regular training and proper handling techniques are essential. The Agilent 1290 Infinity II LC System complies with international safety and environmental standards‚ ensuring adherence to regulatory requirements. Proper documentation‚ including user manuals and maintenance records‚ is essential for GLP compliance. Refer to the manual (Document No: SD-29000230 Rev. G) for detailed specifications and guidelines. Adherence to these standards ensures reliable operation and compliance with local regulations; The Agilent 1290 Infinity II LC System offers advanced features like 2D-LC solutions‚ high-speed pumping‚ and comprehensive analysis capabilities‚ enhancing versatility for complex chromatographic applications and research. The Agilent 1290 Infinity II LC System offers advanced 2D-LC solutions for comprehensive analysis‚ enabling seamless switching between 1D and 2D modes. This enhances separation of complex samples‚ ideal for proteomics and metabolomics. The system’s high-speed pump and precise control ensure optimal performance‚ delivering high-resolution results efficiently. This feature-rich module is designed to meet the demands of advanced chromatographic research and analysis. The Agilent 1290 Infinity II High-Speed Pump delivers exceptional performance with pressures up to 1200 bar‚ enabling rapid and efficient separations. Dual-channel solvent delivery and advanced degassing ensure consistent flow and minimal downtime. Designed for high-speed UHPLC applications‚ this pump supports fast cycle times while maintaining high resolution and sensitivity‚ making it ideal for demanding chromatographic workflows and advanced analytical challenges. The system integrates with InfinityLab software‚ offering advanced control‚ data acquisition‚ and analysis tools. It ensures seamless operation‚ real-time monitoring‚ and compliance with GLP standards. The InfinityLab software provides comprehensive control and analysis for the Agilent 1290 Infinity II LC System. It offers intuitive method development tools‚ advanced data acquisition capabilities‚ and robust data analysis features. The software ensures compliance with GLP standards and supports seamless integration with other modules. Its user-friendly interface enables efficient system operation‚ from sample injection to result interpretation‚ ensuring optimal performance and reproducibility. The Agilent 1290 Infinity II LC System features advanced data acquisition tools within InfinityLab software‚ enabling precise and efficient collection of chromatographic data. Analysis tools include peak detection‚ integration‚ and quantitation‚ with options for custom reporting. The system supports 2D-LC data processing and compatibility with mass spectrometry‚ ensuring comprehensive and accurate results for complex samples. Enhanced visualization and export options facilitate data sharing and interpretation. The Agilent 1290 Infinity II LC System delivers ultra-high performance with a maximum pressure of 1300 bar‚ precise flow rates (0.1-10 mL/min)‚ and advanced temperature control (5-80°C). The Agilent 1290 Infinity II LC System achieves exceptional performance with a maximum pressure of 1300 bar‚ enabling seamless operation across a wide flow rate range (0.1-10 mL/min). It incorporates advanced temperature control (5-80°C)‚ ensuring optimal chromatographic separation. The system’s ultra-low dispersion and high sensitivity make it ideal for challenging analytical applications‚ delivering precise and reliable results consistently. The Agilent 1290 Infinity II LC System requires a controlled environment for optimal operation. It weighs approximately 70 kg and measures 42 cm (width) × 45 cm (depth) × 53 cm (height). Operating conditions include 15-30°C temperature‚ 30-70% humidity‚ and 100-240V AC power. Ensure proper ventilation and adherence to local safety regulations for installation and operation. The Agilent 1290 Infinity II LC System supports various accessories‚ including high-quality chromatography columns‚ sample vials‚ and maintenance kits. Consumables like filters‚ seals‚ and spare parts ensure optimal performance and longevity of the system. Regularly replacing these items is essential for maintaining precision and reliability in chromatographic analysis. The Agilent 1290 Infinity II LC System requires high-purity solvents and reagents to ensure optimal performance. Use LC-MS grade solvents for minimal contamination and consistent results. Avoid additives like salts or ion-pairing agents‚ as they may damage the system. Always follow the recommended solvent compatibility guidelines to maintain instrument integrity and compliance with GLP standards. Regularly replace consumables like columns‚ vials‚ and filters to maintain system performance. Use Agilent-certified parts for compatibility and reliability. Spare parts‚ such as seals and tubing‚ are available through Agilent’s Certified Pre-Owned program. Always refer to the user manual for a comprehensive list of recommended consumables and spare parts to ensure optimal system operation and longevity. The Agilent 1290 Infinity II LC System comes with comprehensive warranty options and dedicated customer support. The Certified Pre-Owned program ensures refurbished modules meet original performance standards. The Agilent Certified Pre-Owned Program ensures refurbished modules meet original performance specifications. Rigorous testing and certification processes guarantee reliability. The program includes a comprehensive warranty and dedicated support‚ providing peace of mind for customers acquiring refurbished Agilent 1290 Infinity II systems. This initiative maintains Agilent’s high standards‚ offering cost-effective solutions without compromising on quality or functionality. Agilent offers comprehensive support options‚ including 24/7 technical assistance and on-site service‚ ensuring optimal system performance. The program provides access to expert engineers‚ online resources‚ and training materials. With a focus on maximizing uptime‚ Agilent’s customer support ensures users can rely on their 1290 Infinity II system for consistent‚ high-quality results. Access Agilent 1290 Infinity II user manuals‚ troubleshooting guides‚ and training materials online. These resources provide in-depth instructions and tips to optimize system performance and user experience. The latest manuals for the Agilent 1290 Infinity II system are available for download from the official Agilent website. These documents include detailed instructions for installation‚ operation‚ and maintenance‚ ensuring users have access to the most up-to-date information to optimize system performance and troubleshooting. Regular updates provide enhancements and new features to support advanced chromatography needs. Agilent provides comprehensive online tutorials and training materials for the 1290 Infinity II system‚ designed to enhance user proficiency. These resources cover operation‚ method development‚ and troubleshooting‚ ensuring scientists and technicians can maximize system capabilities. Accessible through the Agilent website‚ these materials support continuous learning and optimal instrument utilization for achieving high-quality chromatographic results efficiently. The Agilent 1290 Infinity II LC System exemplifies advanced chromatography technology‚ delivering exceptional performance and versatility for modern analytical challenges. Its innovative design and robust capabilities ensure precise results‚ making it an indispensable tool in laboratories worldwide. Agilent continues to redefine chromatography standards with cutting-edge solutions and dedicated support. The Agilent 1290 Infinity II LC System is a benchmark for UHPLC performance‚ offering 1300 bar operating pressure for exceptional resolution and speed. Its modular‚ stackable design maximizes flexibility‚ while advanced detectors like DAD and VWD ensure precision. The system supports high-throughput workflows and integrates seamlessly with InfinityLab software for efficient data acquisition and analysis‚ making it ideal for pharmaceutical‚ biotech‚ and research applications. Agilent continues to innovate‚ with plans to enhance the 1290 Infinity II LC System by integrating next-gen detectors and AI-driven software for smarter workflows. Future updates may include improved sustainability features and expanded compatibility with emerging chromatographic techniques. Additionally‚ Agilent’s Certified Pre-Owned program ensures long-term system viability‚ offering cost-effective access to cutting-edge technology for laboratories worldwide.2.5 MS Flow Modulator (G7170B)
Installation and Setup
3.1 Site Requirements and Specifications
3.2 Step-by-Step Installation Guide
Maintenance and Troubleshooting
4.1 Routine Maintenance Procedures
4.2 Common Issues and Solutions
Operation and Method Development
5.1 Running the System: A Quick Reference
5.2 Developing and Optimizing Chromatographic Methods
Safety and Compliance
6.1 Safety Precautions and Handling Guidelines
6.2 Regulatory Compliance and Documentation
Advanced Features and Applications
7.1 2D-LC Solutions and Comprehensive Analysis
7.2 High-Speed Pump Capabilities
Software and Data Acquisition
8.1 InfinityLab Software Overview
8.2 Data Acquisition and Analysis Tools
Specifications and Technical Details
9.1 Performance Specifications
9.2 Physical and Environmental Requirements
Accessories and Consumables
10.1 Recommended Solvents and Reagents
10.2 Consumables and Spare Parts
Warranty and Support
11.1 Agilent Certified Pre-Owned Program
11.2 Customer Support and Service Options
UserManuals and Additional Resources
12.1 Downloading the Latest Manuals
12;2 Online Tutorials and Training Materials
13.1 Summary of Key Features and Benefits
13.2 Future Developments and Updates